Methods Development

The MLC contributes to the development of new techniques and research methods in areas such as animal husbandry and welfare, phenotyping of mutant lines, and mouse pathology. We are also at the forefront of improvements to technologies such as CRISPR and the latest imaging techniques.

Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods

McCabe, C. V., Price, P. D., Codner, G. F., Allan, A. J., Caulder, A., Christou, S., Loeffler, J., Mackenzie, M., Malzer, E., Mianné, J., Nowicki, K. J., O'Neill, E. J., Pike, F. J., Hutchison, M., Petit-Demoulière, B., Stewart, M. E., Gates, H., Wells, S., Sanderson, N. D., Teboul, L.

(2024) , PLoS Genet , 20 , e1011187

10.1371/journal.pgen.1011187

PMC10954187

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Multi-Photon Microscopy

Sanderson, J.,

(2023) , Curr Protoc , 3 , e634

10.1002/cpz1.634

36706245

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Longitudinal home-cage automated assessment of climbing behavior shows sexual dimorphism and aging-related decrease in C57BL/6J healthy mice and allows early detection of motor impairment in the N171-82Q mouse model of Huntington’s disease

Bains, R. S., Forrest, H., Sillito, R. R., Armstrong, J. D., Stewart, M., Nolan, P. M., Wells, S. E.

(2023) , Front Behav Neurosci , 17 , 1148172

10.3389/fnbeh.2023.1148172

PMC10073658

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Genotyping Genome-Edited Founders and Subsequent Generation

Mackenzie, M., Fower, A., Allan, A. J., Codner, G. F., Bunton-Stasyshyn, R. K., Teboul, L.

(2023) , Methods Mol Biol , 2631 , 103-134

10.1007/978-1-0716-2990-1_4

36995665

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Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions

Chenouard, V., Leray, I., Tesson, L., Remy, S., Allan, A., Archer, D., Caulder, A., Fortun, A., Bernardeau, K., Cherifi, Y., Teboul, L., David, L., Anegon, I.

(2023) , iScience , 26 , 106399

10.1016/j.isci.2023.106399

PMC10074149

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