
Conditional Transgenics
Transgenic mice are an important resource to understand gene function, regulation, and expression. This course is designed to introduce the principles behind conditional genetic modifications, experimental. design and analysis
Who is it for?
- Animal Facility Managers
- Researchers with previous knowledge of advanced mouse genetics
After the course you will be able to:
- Understand the basic principles of conditional mutagenesis
- Identify the advantages and challenges of these systems
- Plan for restricting the expression of a transgene
- Analyse recombinase (Cre) expression
Recommended level of previous knowledge required for this course: Advanced Mouse Genetics
Hourly Schedule
- 10:00 - 10:30
- Introductions
- Registration, Orientation, Coffee, Icebreaker and H&S
- 10:30 - 12:30
- Session 1
- Conditional systems: Principles, building cre expressors and checking their activity
- 12:30 - 13:00
- Lunch
- 13:00 - 13:45
- Session 2
- Working with conditional and inducible transgenic mice
- 13:45 - 14:00
- Tea and coffee break
- 14:00 - 16:00
- Session 3
- Breeding conditional transgenics for analysis: worked example and workshop
- 16:00 - 17:00
- Tour (optional)
- Mary Lyon Centre